Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep

Figure 7

miR-125a-5p downregulates the expression of ESRRα by targeting its 3′ UTR site. (a) The pairing schematic of ESRRα 3′ UTR and miR-125a-5p. The nucleotide in blue represents “seed sequence” of miR-125a-5p, and the mutation nucleotides are presented in red. (b) The insertion site of ESRRα 3′ UTR or its mutation luciferase reporter vector map. (c) The two types were cotransfected with miR-125a-5p mimics (or negative control) into 293T cells. Luciferase assay was performed 48 h after transfection. Results are presented as relative luciferase activity (mean ± SD; ; ANOVA; ).

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