BioMed Research International / 2017 / Article / Fig 5

Research Article

MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7

Figure 5

Effects of miR-195 on α-SMA and Smad7 in HSC-T6 treated with TGF-β1 (5 ng/ml) for 24 h, 48 h, and 72 h. (a) miR-195 expression was determined in HSC-T6 transfected with miR-195 mimic (100 nM) or miR-195 inhibitor (200 nM) by qRT-PCR. (b) α-SMA mRNA expression was determined in HSC-T6 transfected with miR-195 mimic (100 nM) or miR-195 inhibitor (200 nM) by qRT-PCR. (c) Smad7 mRNA expression was determined in HSC-T6 transfected with miR-195 mimic (100 nM)/inhibitor (200 nM) by qRT-PCR. (d) Smad7 protein expression was determined in HSC-T6 transfected with miR-195 mimic (100 nM)/inhibitor (200 nM) by Western blot. Each value represents the mean ± SD of three experiments. , versus control; versus TGF-.
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