The Neuroprotective Effects of Muscle-Derived Stem Cells via Brain-Derived Neurotrophic Factor in Spinal Cord Injury Model

Figure 2

Effects of MDSCs on neurons. (a) The neurons stained with Hoechst 33258. Arrowheads (▲) indicate normal neurons and arrows (↑) indicate apoptotic neurons. Five fields were randomly selected and the (b) apoptotic rates were measured. (c) The ratio of apoptosis of neurons was measured by Annexin V-FITC/propidium iodide double staining. Data was shown as mean ± SEM (), versus NC group, and versus NT group. NC (no treatment); NT (neurons treated with t-BHP); MNT (neurons cocultured with MDSCs after treatment with t-BHP). Scale bar: 100 μm.