Immune Response and Protective Efficacy of a Heterologous DNA-Protein Immunization with Leishmania Superoxide Dismutase B1

Figure 6

Antigen-specific antibody response in BALB/c mice after L. major infection. LdFeSODB1-specific total IgG (a), IgG1 (b), IgG2a (c), and IgG2a/IgG1 ratio; (d) antibody response after infection with L. major. The antibody response was measured using ELISA and the result is depicted as mean of five mice per group and standard error of the mean (SEM). The assay was done in duplicate wells for each mouse serum. Point 0 indicates the time when the mice were infected with L. Major. The statistical difference was compared using compared using Mann–Whitney test. Asterisks indicate statistically significant difference in IgG2a/IgG1 ratio between mice immunized with the antigen and the control groups ().

(a)

(b)

(c)

(d)