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BioMed Research International
Volume 2017 (2017), Article ID 2152749, 7 pages
Research Article

In Vitro Antibacterial Activity of Pomegranate Juice and Peel Extracts on Cariogenic Bacteria

1Department of Neuroscience, Reproductive and Oral Sciences, Section of Paediatric Dentistry, University of Naples Federico II, Via S. Pansini, No. 5, 80131 Naples, Italy
2Department of Science and Technology, Sannio University, Via Port’arsa, No. 11, 82100 Benevento, Italy
3Department of Molecular Medicine and Medical Biotechnology, Federico II University Medical School, Via S. Pansini, No. 5, 80131 Naples, Italy
4CEINGE, Advanced Biotechnologies, Via Gaetano Salvatore, No. 486, 80145 Naples, Italy
5Institute of Food Science-CNR, Via Roma, No. 64, 83100 Avellino, Italy

Correspondence should be addressed to Gianmaria Fabrizio Ferrazzano

Received 23 July 2017; Accepted 2 October 2017; Published 25 October 2017

Academic Editor: Yiannis Kourkoutas

Copyright © 2017 Gianmaria Fabrizio Ferrazzano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. To evaluate the antimicrobial activity of hydroalcoholic extracts of pomegranate (Punica granatum L.) peel and juice, against the microorganisms considered the main etiologic agents of dental caries. Methods. The values of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined against Streptococcus mutans Clarke ATCC® 25175™ strain and Rothia dentocariosa clinical isolate. Results. Peel extracts inhibit effectively the growth and survival of S. mutans ATCC 25175 strain and R. dentocariosa clinical isolate with MIC and MBC values of 10 μg/μl and 15 μg/μl, respectively. Furthermore, the pomegranate juice extract showed high inhibitory activity against S. mutans ATCC 25175 strain with a MIC value of 25 μg/μl and a MBC value of 40 μg/μl, whereas, against R. dentocariosa, it has displayed a moderate inhibitory activity, with MIC and MBC values of 20 μg/μl and 140 μg/μl, respectively. Conclusions. In vitro microbiological tests demonstrate that the hydroalcoholic extracts of pomegranate juice and peel are able to contrast the main cariogenic bacteria involved in tooth decay. Although being preliminary data, our results suggest that pomegranate polyphenolic compounds could represent a good adjuvant for the prevention and treatment of dental caries.