Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators

Table 1

Primer sequence.

Gene	Primer sequence (5′-3′)	Annealing temperature (°C)
Cyclin B1	F: AATAAGGCGAAGATCAACATGGC	58
	R: TTTGTTACCAATGTCCCCAAGAG
Cyclin D1	F: GCTGCGAAGTGGAAACCATC	58
	R: CCTCCTTCTGCACACATTTGAA
Cyclin E1	F: GCCAGCCTTGGGACAATAATG	58
	R: CTTGCACGTTGAGTTTGGGT
p21	F: TGTCCGTCAGAACCCATGC	58
	R: AAAGTCGAAGTTCCATCGCTC
p53	F: CCCCTCCTGGCCCCTGTCATCTTC	58
	R: GCAGCGCCTCACAACCTCCGTCAT
β-Actin	F: CATGTACGTTGCTATCCAGGC	58
	R: CTCCTTAATGTCACGCACGAT
GAPDH	F: GAGTCAACGGATTTGGTCGT	58
	R: TTGATTTTGGAGGGATCTCG