Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators

Table 2

Impact of four small molecules and their combinations on megakaryocyte maturation judged by K562, UT-7, MEG-01 cells, and primary MK progenitors (MK-PROs).

	NIC	RRI	SI	ABI	RRI + SI	RRI + NIC	RRI + SI + NIC
Cell diameter	—	↑^1	↑	↑^2	↑	↑	↑^3
DNA ploidy	—^4	—	↑	↑	↑	—^5	↑
Proliferation	—	—	—	—^6
CD61 expression^7	—	—	—	↓	↑	—	↑
Apoptosis^7	↑	—	—	↑	—	↑	↑

or ABI did not increase the cell diameter of MK-PROs; + SI + NIC did not increase the cell diameter of MK-PROs and UT-7 cells; alone or with SI reduced the percentage of highly polyploid cells in UT-7 cells; -treatment resulted in cell amplification blockage in MK-PROs; expression and apoptosis were summarized from the result of MK-PROs.