Automated Image Analysis of HER2 Fluorescence In Situ Hybridization to Refine Definitions of Genetic Heterogeneity in Breast Cancer Tissue

Figure 4

Examples of the tumor cases from the clusters extracted from the automated HER2 FISH data. Histograms of HER2, CEP17, and HER2/CEP17 with Gaussian curves are presented from the cases labeled in the Figure 3. Amplification, polysomy, and genetic heterogeneity categories are based on the conventional manual procedure results. Bim_HER2, Bim_CEP17, and Bim_Ratio represent bimodality categories based on Ashman’s D > 2 criterion.

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