Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

Figure 2

C4.4A in the development of human tissue-engineered skin. (a–g) Expression of C4.4.A (green) in TES at days 1 (a), 2 (b), 3 (c), 4 (d), 7 (e), 9 (f), and 11 (g, h) of culture at the A/L interface. (a′), (b′), (c′), (d′), (e′), (f′), and (g′): Phase contrast images corresponding to panels (a), (b), (c), (d), (e), (f), and (g) respectively. (h) Negative control (omission of the primary antibody). Cell nuclei are stained with Hoechst (blue). A/L, air-liquid; d, day; sb, stratum basale; ss, stratum spinosum; sg, stratum granulosum; sc, stratum corneum; TES, tissue-engineered skin. Scale bar: 25 μm.