BioMed Research International / 2017 / Article / Fig 3

Research Article

Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

Figure 3

C4.4A expression in relation to epidermal differentiation markers. (a) Expression of C4.4A (green) and K10 (red) in TES at day 10 of culture at the A/L interface. K10 labeling is also shown separately in panel (b). (d) Expression of C4.4A (red) and K14 (green) in TES at day 11 of culture at the A/L interface. (f) Expression of C4.4A (green) and the basement membrane marker laminin-5 (red) in TES at day 11 of culture at the A/L interface. (g–i) Expression of C4.4A (red) and Haldisin (green) in TES at days 6–8 of culture at the A/L interface. (c and e) Phase contrast images corresponding to panels (b) and (d), respectively. Cell nuclei are stained with Hoechst (blue). The difference in K10 and K14 expression in the basal layer is emphasized by asterisks in panels (a), (b), and (d). The localization of the basement membrane, separating the fibroblast sheets of the dermal portion of the TES from the keratinocytes of the epidermal compartment is stressed by a dotted line in panels (a), (b), (c), (e), (g), (h), and (i). A/L, air-liquid; K, keratin; sb, stratum basale; ss, stratum spinosum; sg, stratum granulosum; sc, stratum corneum; TES, tissue-engineered skin. Scale bar = 50 μm.

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