Extremely Low-Frequency Electromagnetic Fields Affect Myogenic Processes in C2C12 Myoblasts: Role of Gap-Junction-Mediated Intercellular Communication

Figure 3

Expression levels of MyoD and myogenin in differentiating C2C12 myoblasts during ELF-EMF treatments. ((a) and (c)) Representative immunoblot of MyoD and myogenin expression levels, respectively. ((b) and (d)) Densitometry analysis of MyoD and myogenin expression levels, respectively, plotted as relative expression calculated by the ratio between OD × mm² of MyoD or myogenin band found in ELF-EMF-treated cells (0.1 mT, 0.5 mT, or 1.0 mT) and OD × mm² of the respective band in the corresponding control (Ctr). Data are means ± SEM from three independent experiments. and versus corresponding Ctr.