BioMed Research International / 2017 / Article / Fig 2

Research Article

The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells

Figure 2

The effects of extracts from light-curable pulp capping materials (TC and BA) as well as the gold standard pulp capping material (DC) on alkaline phosphatase (ALP) activity (at 14 and 21 days) (a) and on calcium deposition using alizarin red staining (ARS) (at 21 days) (b and c). After ARS was observed under the microscope (c), 10% w/v cetylpyridinium chloride monohydrate was used for quantitative analysis (b). The cells were incubated for 7 to 21 days with 12.5% diluted extract in odontogenic supplemental medium (OM) for the differentiation of hDPSCs or supplemented media only for the negative control. Different letters indicate significant differences among the groups at . The measurements were performed in triplicate, and representative averages () with SD or images are presented.
(a)
(b)
(c)

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