BioMed Research International / 2017 / Article / Fig 3

Research Article

The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells

Figure 3

The ions released from the extracts of pulp capping materials as detected by inductively coupled plasma atomic emission spectrometry analysis (a) and pH measurement (b). (c and d) After an ionic supplement including the ions that were differentially released between the TC and BA extract was added to the BA extract to match the ionic conditions, the ionic supplement-added 12.5% BA extract was cultured with odontogenic supplemental medium (OM) for 21 days to compare the biological effects of 12.5% TC, BA, and ionic supplement. Calcium deposition was measured using alizarin red staining (ARS). After ARS was observed under the microscope (c), 10% w/v cetylpyridinium chloride monohydrate was used for quantitative analysis (d). The measurements were performed in triplicate, and representative averages () with SD or images are presented. Different letters indicate significant differences among the groups at .

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