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BioMed Research International
Volume 2017, Article ID 2564363, 10 pages
https://doi.org/10.1155/2017/2564363
Research Article

Analysis of the Metabolites of Indole Degraded by an Isolated Acinetobacter pittii L1

School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China

Correspondence should be addressed to Junhui Zhou; nc.ude.tudg.2liam@0111125smc

Received 27 August 2017; Accepted 19 November 2017; Published 13 December 2017

Academic Editor: Shijian Ge

Copyright © 2017 Zuoyi Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human and ecological environment. In this study, a Gram-negative bacterial strain of eliminating indole was isolated from a coking wastewater. The strain was confirmed as Acinetobacter pittii L1 based on the physiological and biochemical characterization and 16S ribosomal DNA (rDNA) gene sequence homology. 400 mg/L indole could be completely removed within 48 h by the strain on the optimum condition of 37°C, pH 7.4, and 150 rpm. The organic nitrogen was converted to NH3-N and then to and the organic carbon was partially transferred to CO2 during the indole biodegradation. The metabolic pathways were proposed to explain the indole degradation based on the liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of indigo, 4-(3-Hydroxy-1H-pyrrol-2-yl)-2-oxo-but-3-enoic acid, and isatin. The toxicity of the biodegradation products was evaluated using the Microtox test, which revealed that the metabolites were more toxic than indole. Our research holds promise for the potential application of Acinetobacter pittii L1 for NHCs degradation, production of indigoids, and soil remediation as well as treatment of indole containing wastewater.