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BioMed Research International
Volume 2017, Article ID 2597256, 9 pages
https://doi.org/10.1155/2017/2597256
Research Article

Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

1Department of Histology and Embryology, Guizhou Medical University, Guiyang, Guizhou 550004, China
2People’s Hospital in Yueyanglou District, Yueyang, Hunan 414000, China
3Department of Histology and Embryology, Zunyi Medical College, Zunyi, Guizhou 563000, China

Correspondence should be addressed to Gao Xiao-Qin; moc.anis@103055qxg

Received 27 December 2016; Revised 9 April 2017; Accepted 14 May 2017; Published 16 July 2017

Academic Editor: Ping-Ho Chen

Copyright © 2017 Dai Yan-Ping et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To study the expressions of VEGF and VEGFR2 at protein level in the epididymis of rats with arsenism. Methods. Forty male Sprague-Dawley rats were randomly divided into four groups: the high dose arsenic infected group (60.0 mg/L in water), the middle dose arsenic infected group (12.0 mg/L in water), the low dose arsenic infected group (2.4 mg/L in water), and the control group (distilled water). Rats were treated with arsenic through drinking water for 6 consecutive months. At the end of the experiment, the average densitometry values of apoptotic cells in epididymis tubules were determined by TUNEL method; the protein and mRNA levels of VEGF and VEGFR2 were observed by immunohistochemistry, Western blot, and real time fluorescent quantitative PCR, respectively. Results. Compared with the control group, in each infected group, the average densitometry values of apoptotic cells in the epididymis tubules were significantly lower. Compared with control group, protein and mRNA levels of VEGF and VEGFR2 in each infected group were obviously declined. The correlations between protein and mRNA levels of VEGF and VEGFR2 were positively exhibited (r = 0.843, 0.869, p < 0.05). Conclusions. Arsenism affects the expressions of VEGF and VEGFR2 in the epididymis of rats and results in apoptosis of pathophysiology of male infertility.