miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2/PI3K Pathway

Figure 1

miR-200c promotes the activity, proliferation, and migration of HSCs. (a, b) Construction of two cell lines (LX2-200c and LX2-nc) from the human HSC line (LX2). LX2-200c stably overexpressed miR-200c and LX2-nc expressed empty vector as the control. Green fluorescent protein (GFP) expression was observed in LX2-200c and LX2-nc by fluorescence microscopy, rather than in LX2 (original magnification ×400). The level of miR-200c expression in LX2-200c was approximately 314 times of that in LX2-nc cells. (c) LX2-200c had markedly higher levels of α-SMA and vimentin. (d) miR-200c promoted the proliferation of LX2. (e, f) The migration of LX2 was increased by miR-200c. means , means , and means .

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