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BioMed Research International
Volume 2017, Article ID 2782850, 13 pages
Research Article

Efficacies of Various Anaerobic Starter Seeds for Biogas Production from Different Types of Wastewater

1Biotechnology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkhunthian, Bangkok 10150, Thailand
2ECoWaste, The Joint Graduate School of Energy and Environment, King Mongkut’s University of Technology Thonburi, Thungkru, Bangkok 10140, Thailand

Correspondence should be addressed to Pawinee Chaiprasert;

Received 30 May 2017; Revised 13 July 2017; Accepted 24 July 2017; Published 28 August 2017

Academic Editor: Pengjun Shi

Copyright © 2017 Pawinee Chaiprasert et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Various anaerobic starter seeds from different sources were investigated for their efficacies in treatment of different types of wastewater. Six combinations of starter seeds and wastewaters were selected out of 25 combination batch experiments and operated in semicontinuous reactors. It was noticed that the efficacies of various anaerobic starter seeds for biogas production from different types of wastewater in terms of reactor performance and stability were depended on wastewater characteristics and F/M ratio affecting microbial community and their microbial activities. However, exogenous starter seed can be used across different types of wastewater with or without acclimatization. Four reactors reached the targeted OLR of 2 kg COD/m3·d with high performance and stability except for concentrated rubber wastewater (RBw), even using high active starter seeds of cassava starch (CSs) and palm oil (POs). The toxic compounds in RBw such as ammonia and sulfate might also adversely affect methanogenic activity in CSsRBw and POsRBw reactors. DGGE analysis showed that propionate utilizers, Smithella propionica strain LYP and Syntrophus sp., were detected in all samples. For Archaea domain, methylotrophic, hydrogenotrophic, and acetoclastic methanogens were also detected. Syntrophic relationships were assumed between propionate utilizers and methanogens as acetate/H2 producers and utilizers, respectively.