Efficacies of Various Anaerobic Starter Seeds for Biogas Production from Different Types of Wastewater

Table 6

DGGE profiles of Archaea domain in initial starter seeds and six selected combinations.

Archaea group	Closest match	Similarity (%)	Accession number	Initial starter seeds	Start-up period
					CSsSWw	CSsRBw	POsRBw	RBsSMw	SMsPOw	SWsCSw
Methylotrophic methanogens	Methanosarcina acetivorans strain C2A	80	NR_074110.1			+ (3)	+ (3)	+ (3)	+ (3)
	Methanomethylovorans hollandica strain DSM 15978	93	NR_102454.1	All (band 8)	+ (7)	+ (7)	+ (7)	+ (7)	+ (7)	+ (7)
	Methanomethylovorans hollandica strain DMS1	99	NR_028174.1					+ (9)	+ (9)
Hydrogenotrophic methanogens	Methanolinea mesophila strain TNR	86	NR_112799.1		+ (4)
	Methanolinea tarda strain NOBI-1	86	NR_028163.1		+ (5)	+ (5)	+ (5)	+ (5)	+ (5)	+ (5)
	Methanoregula formicica strain SMSP	82	NR_102441.1	CSs, SWs (band 9)		+ (6)	+ (6)	+ (6)	+ (6)	+ (6)
Acetoclastic methanogens	Methanosaeta concilii strain GP6	89	NR_102903.1	All (band 2)	+ (1, 2, 8, 11)	+ (1, 2, 8, 11)	+ (1, 2, 8, 11)	+ (1, 2, 8, 11)	+ (1, 2, 8, 11)	+ (1, 2, 8, 11)
	Methanosaeta concilii strain Opfikon	94	NR_028242.1	CSs (band 3)
	Methanosarcina mazei Go1	98	NR_074221.1			+ (10)	+ (10)
	Methanosaeta harundinacea	99	NR_102896.1	All (band 11)
Another Archaea	Uncultured methanogenic archaeon	88	HQ141845.1	CSs, SWs (band 1)
	Methanogenic prokaryote enrichment culture	99	KC821320.1	All (band 4)
	Uncultured archaeon clone	91	EF639564.1	All (band 5)
	Uncultured Methanobacteriaceae archaeon	92	GU982677.1	All (band 6)
	Uncultured Methanosaeta sp.	99	AY454766.1	POs (band 7)
	Uncultured Methanosaeta sp.	99	AY454766.1	SMs (band 10)

(Number) = band in DGGE analysis and + (number) = the presence of microorganism in DGGE band.