Interleukin-9 Promotes Pancreatic Cancer Cells Proliferation and Migration via the miR-200a/Beta-Catenin Axis

Figure 3

miR-200a target luciferase reporter assay. (a) Reporter construct containing psiCHECK2-WT-β-catenin 3′-UTR and psiCHECK2-MUT-β-catenin 3′-UTR was cotransfected into HEK293T cells. Relative luciferase activity was normalized to firefly luciferase. Luciferase reporter assay revealed that the relative luciferase level was significantly decreased in cells transfected with miR-200a, suggesting that β-catenin was a target of miR-200a. (b) Comparison of β-catenin protein levels in miR-200a mimics infection group (miR-200a mimics) and negative control group (NC) in PANC-1 cells; the expression of β-catenin protein levels was decreased after infecting miR-200a mimics. (c) Western-blot result of β-catenin in PANC-1 cells; GAPDH was used as a control. (d) Comparison of β-catenin protein levels in miR-200a mimics infection group (miR-200a mimics) and negative control group (NC) in AsPC-1 cells; the expression of β-catenin protein levels was decreased after infecting miR-200a mimics. (e) Western-blot result of β-catenin in AsPC-1 cells; GAPDH was used as a control. Data was expressed as mean ± SD. .

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