Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro
RLX alleviates the ioversol induced cytotoxicity in HK-2 cells. We further examined the effect of H2-RLX on ioversol (100 mg/mL) treated HK-2 cells at 24 h using a CCK-8 assay. The concentrations employed were based on the previous results. HK-2 cells were pretreated with RLX (10 ng/mL) and LY294002 (50 μM) for 1.5 hours before ioversol was added. And then cells were exposed to ioversol (100 mg iodine/mL) for 30 minutes, followed by further incubation for 24 hours in the absence of ioversol. Data are reported as the mean ± SD; .