BioMed Research International / 2017 / Article / Fig 3

Research Article

Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro

Figure 3

RLX reduces ioversol induced apoptosis. HK-2 cells were pretreated with RLX (10 ng/mL) and LY294002 (50 μM) for 1.5 hours and then coincubated with ioversol (100 mg iodine/mL) for 30 minutes, followed by further incubation for 24 hours in the absence of ioversol. Cell apoptosis was analyzed by flow cytometry using Annexin V-FITC and PI staining. Data showed a marked increase of apoptotic cells in the ioversol treated cells; however, apoptotic cells were reduced after treatment with RLX; this effect was inhibited by the PI3K/Akt pathway inhibitor LY 294002.