HPV16 E6 Promotes Breast Cancer Proliferation via Upregulation of COX-2 Expression

Figure 1

HPV16 E6 promotes the proliferation of breast cancer. (a) The expression of HPV16 E6 in MCF-7/HPV16 E6 and MCF-7/Vector by Western blot. (b)–(d) The proliferative ability of the indicated cells detected by MTT assays, colony formation assays, and soft agar assays. Only cell colonies containing more than 50 cells were counted. Error bars represent mean ± SD from 3 independent experiments. . (e) MCF-7/HPV16 E6 and MCF-7/Vector cells were injected into the hind limbs of nude mice (). Tumor volumes were measured on the indicated days. The tumor volume data were presented as the mean ± SD. (f) Histopathological analyses of xenograft tumor. The tumor sections were stained with H&E or subjected to IHC staining using an antibody against Ki-67. Error bars represent mean ± SD from three independent experiments. .

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