Role of Phosphorylated HDAC4 in Stroke-Induced Angiogenesis

Figure 3

Phosphorylated HDAC4 regulates angiogenesis in vitro. (a) RBMEC cells treated with GÖ6976 or vehicle control were cultured in normoxic condition (21% O₂, 5% CO₂) or under hypoxia (1% O₂, 5% CO₂, and 94% N₂) for 12 or 24 hours. CCK-8 cell proliferation analysis was performed at the indicated time. ((b) and (c)) Tube formation assay was assessed 12 hours after treatment with or without GÖ6976. Representative images are shown in (b), and total loops are quantified in (c). ((d) and (e)) Wound scratch assay was analyzed at the indicated time. Representative images are shown in (d), and migration areas are quantified in (e). Data are presented as mean ± SD. N: cells cultured in normoxic condition; H: cells cultured in hypoxia condition. versus N; versus H. Scale bar = 250 μm.

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