Research Article

Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enhance BMP-2 Production

Figure 6

Analysis of calcification in hUCB-MSC cultures treated with PEI, PEI25, or mTAT/PEI complexes of gWiz-BMP2. Transfection with complexes was carried out for 24 hours, after which the medium was changed to OM. The extent of calcification was quantified spectroscopically at 14 and 21 days after transfection. (a) Representative Alizarin Red-stained cultures. (b) Quantitative analysis of calcium deposition. versus NT. versus PEI or PEI25.
(a)
(b)