Purification and Characterization of an ATPase GsiA from Salmonella enterica

Figure 6

Effects of GsiA on glutathione uptake. gsiA and ggt gene deletion strains were constructed. The glutathione uptake curves of mutant and wild type Salmonella enterica were analyzed. M9 medium was used as minimal medium with MgSO₄ replaced by MgCl₂. Reduced glutathione (1 mM, ≥98%) was served as the only sulfur source. Glutathione concentration in the medium was measured by Glutathione Assay Kit (Sigma) according to the instruction manual.