Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types

Figure 5

TEM micrographs of PDBs effect on MDA-MB-231 cell line. (a) TEM micrographs illustrating the effect of PBDs on the intracellular organelles of MDA-MB-231 cell line. PBDs deposits (D) were scattered over the intercellular organelles (N; nucleus, Nu; nucleolus, F; fat droplet, M; mitochondria, ER; endoplasmic reticulum, G; Golgi apparatus, P; phagosome, and V; villi) causing structural changes. (b) TEM micrographs of MDA-MB-231 treated cells with PBDs compared to control. (A) illustrates the ultrastructural change of mitochondria (arrows), (A)(i) untreated (40000x), (A)(ii) 10 μM Cisplatin treated cell for 2 h (60000x), (A)(iii) 20 μM Carboplatin treated cells for 4 h (120000x), and (A)(iv) 20 μM Oxaliplatin treated cell for 4 h (50000x). (B) illustrates chromatin condensation and fragmentation (arrows), (B)(i) untreated (8000x), (B)(ii) 10 μM Cisplatin treated cells for 4 h (6000x), (B)(iii) 10 μM Carboplatin treated cells for 2 h (8000x), and (B)(iv) 10 μM Oxaliplatin treated cell for 4 h (10000x). (C) demonstrates phagosome formation (arrows), (C)(i) untreated (20000x), (C)(ii) 10 μM Cisplatin treated cells for 2 h (25000x), (C)(iii) 20 μM Carboplatin treated cells for 2 h (40000x), and (C)(iv) 20 μM Oxaliplatin treated cell for 2 h (40000x). (c) TEM micrographs represent some cells of MDA-MB-231 with no nucleus (A) while MCF-7 and BT-474 have more than one nucleus (B and C, resp.).

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