Royal Jelly Inhibits Pseudomonas aeruginosa Adherence and Reduces Excessive Inflammatory Responses in Human Epithelial Cells

Figure 3

No cytotoxicity effect of royal jelly extract on pharyngeal, Detroit 562 (a) and lung and NCI-H292 (b), epithelial cells. As a positive control, epithelial cells were treated with 0.1% Triton X-100 and shaken gently for 10 min at room temperature. Epithelial protective effect of royal jelly against 0.1% Triton X-100 (c). Detroit 562 monolayers cultured in a 24-well plate were pretreated with 25% royal jelly or PBS for 30 s at room temperature, washed with PBS twice, and irritated with 0.1% Triton X-100 for 30 sec at room temperature. Data represent the means ± SDs of 3 independent experiments. Asterisks indicate significant differences ( and ) versus control group (0% royal jelly).

(a)

(b)

(c)