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BioMed Research International
Volume 2017, Article ID 3196924, 7 pages
https://doi.org/10.1155/2017/3196924
Research Article

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

1Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka
2Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka
3Dengue Mega Project, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

Correspondence should be addressed to Nayana Gunathilaka; kl.ca.nlk@akalihtanug.n

Received 21 January 2017; Revised 26 May 2017; Accepted 17 July 2017; Published 15 August 2017

Academic Editor: Cheng-Feng Qin

Copyright © 2017 Nayana Gunathilaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors. Methodology. Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken) were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti. Significance in the variations among blood feeding was investigated by one-way ANOVA, cluster analysis of variance (ANOSIM), and principal coordinates (PCO) analysis. Results. Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA (). The metal plate method was identified as the most efficient and cost-effective feeding technique. Blood feeding rate of Ae. aegypti was higher with human blood followed by cattle and chicken blood, respectively. However, no significant difference was observed from the mosquitoes fed with cattle and human blood, in terms of fecundity, oviposition rate, and fertility as suggested by one-way ANOVA (). Conclusions. Metal plate method could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti, due to its high feeding rate and cost effectiveness. Cattle blood could be recommended for mass rearing Ae. aegypti.