Reduction of Mosquito Survival in Mice Vaccinated with Anopheles stephensi Glucose Transporter

Figure 2

Fluorescence Microscopy of A. stephensi-Infected Salivary Glands Triple-Stained. Salivary glands were incubated with PBS, preimmune and anti-GTp immune serum. Green fluorescence corresponds to GFP-expressing P. berghei, blue to the nuclei stained with DAPI and magenta for Cy5-Alexa Fluor 647 to localize specific anti-GTp antibodies. The fluorescence intensity of SG Z-stack and merged images was compared and then analyzed in the same conditions. A specific and localized recognition was evident when SGs were incubated with the anti-GTp serum. Z-stack maximum intensity projections and a merge image of the three channels are given. Green: GFP-expressing P. berghei. Blue: DAPI-counterstaining of the nuclei. Magenta: Cy5-Alexa Fluor 647. Bar: 25 µm.