Reduction of Mosquito Survival in Mice Vaccinated with Anopheles stephensi Glucose Transporter

Figure 3

Schematic Overview of Vaccination Trial. From twelve five-week-old female Balb/c mice, six were primed with PBS and the others with GTp-formulated vaccine. Every fortnight, mice were boosted five times intraperitoneally. Four days after the last immunization, mice were infected with P. berghei parasitized red blood cells by intraperitoneal inoculation or left uninfected as control. Blood samples were collected to determinate antibody titers during immunization and after Plasmodium infection. For five days the infection was monitored and when the parasitaemia reached 10–20% and 4–6 exflagellations/field were observed, mice were anesthetized and used to feed and infect female mosquitoes (). Mosquito survival and oviposition were assessed until the 18th day after blood meal (PBM).