(a)
(b)
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Figure 4: Psoralen regulated the IRE1-ASK1-JNK pathway in osteoporotic osteoblasts. (a, e, and g) Osteoporotic osteoblasts were pretreated by TM, AM, or DMSO for 1 h, and then osteoblasts were incubated with psoralen or E2 for 48 h. Then protein expression was determined by western blot, and (d and f) mRNA expression was determined by qPCR. (b and c) Osteoporotic osteoblasts were pretreated by TM or AM for 1 h. Then the cells were incubated with psoralen for 48 h. The cell viability was determined by MTT assay. , , .