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BioMed Research International
Volume 2017, Article ID 3620510, 14 pages
https://doi.org/10.1155/2017/3620510
Research Article

Recombinant Vaccinia Viruses Coding Transgenes of Apoptosis-Inducing Proteins Enhance Apoptosis But Not Immunogenicity of Infected Tumor Cells

1Department of Biotechnology, Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia
2Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia
3Department of Viral Hepatitis, State Research Center of Virology and Biotechnology “Vector”, Rospotrebnadzor, Koltsovo, Russia

Correspondence should be addressed to Olga Koval; ur.sgn@lavok_o

Received 25 April 2017; Revised 17 July 2017; Accepted 26 July 2017; Published 30 August 2017

Academic Editor: Steven De Vleeschouwer

Copyright © 2017 Olga Koval et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

S1 Fig. Visualization of lytic (apoptotic) morphology of infected MX-7 cells. Cells were infected with VV-GMCSF-dGF, VV-GMCSF-Lact and VV-GMCSF-Apo. After 24 and 48 h post-infection, cells were analyzed by inverted microscope (phase-contrast) and photographed. Representative photomicrographs of three independent experiments. S2 Fig. Visualization of lytic (apoptotic) morphology of infected MCF-7 cells. Cells were infected with VV-GMCSF-dGF, VV-GMCSF-Lact and VV-GMCSF-Apo. After 6, 24 and 48 h post-infection, cells were analyzed by inverted microscope (phase-contrast) and photographed. Representative photomicrographs of three independent experiments. S3 Fig. Mitochondrial apoptosis activation in MX-7 cells. Cells were treated with VVs (0.05 PFU/cell). (A) Fluorescence microscopy of infected cells treated with JC-1 indicator. (B) Flow cytometry analysis of VV-infected cells stained with JC-1.

  1. Supplementary Material