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BioMed Research International
Volume 2017, Article ID 3769245, 7 pages
Research Article

Microcystins Presence in Mussels (M. galloprovincialis) and Water of Two Productive Mediterranean’s Lagoons (Sardinia, Italy)

Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy

Correspondence should be addressed to Valeria Pasciu; ti.ssinu@uicsapv

Received 28 June 2017; Revised 15 September 2017; Accepted 1 November 2017; Published 21 November 2017

Academic Editor: Miroslav Pohanka

Copyright © 2017 Elena Baralla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Microcystins (MCs) are hepatotoxins harmful for animal and human health. The most toxic type between them is MC-LR whose presence has been investigated in different reservoirs all around the world. In this work microcystins were monitored in spring and summer in water and mussels (Mytilus galloprovincialis) of two Sardinia lagoons: Cabras and Calich lagoons. A Solid Phase Extraction method was developed to clean and concentrate samples before the Enzyme Linked Immunosorbent Assay (ELISA) and the following Mass Spectrometry detection. MCs presence was detected using the screening ELISA test in both lagoons. MCs peak was revealed in July for water and mussels belonging to Cabras lagoon ( ng/L in water and  ng/g ww in mussels). In water of Calich lagoon there was a constant trend in the concentration of MCs during the considered months, while there was a MCs peak in July ( ng/g ww) in mussels. The following LC-MS/MS analysis did not reveal MC-LR presence in all analyzed samples. These results can be useful to enrich knowledge on public health and consumer’s safeguard.