Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways
SIRT1 is strongly upregulated in the BCa tissues compared with both paracancerous tissues and normal bladder tissues. (a) qRT-PCR analysis exhibited that the gene expression of SIRT1 in bladder cancer tissues was significantly higher than the matched paracancerous tissues. The value of GAPDH was used as an internal control. . (b) Representative result of double immunofluorescence staining of SIRT1 (green) in bladder cancer tissues (A, D), paracancerous tissues (B, E), and normal bladder tissues (C, F). The OCT4 (red) was stained as a marker of BCa cells. Nuclei (blue) were stained by DAPI. The scale bar for (b) is 100 μm. (c) Overrepresentation analysis using microarray raw data and DAVID database suggested that the FOXO signaling pathway and related genes were affected (red) in the BCa tissues (FOXO signaling pathway, map04068, KEGG pathway image, copyright permission obtained from KEGG).
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