Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways

Table 3

List of primary antibodies.

Antigens	Species antibodies raised in	Dilution (IF)	Dilution (WB)	Supplier
Acetyllysine, acetylated KLH conjugates	Rabbit, polyclonal	—	1 : 500	Abcam, UK, Cat. #ab80178
Catalase, human	Rabbit, monoclonal	—	1 : 2,000	Abcam, UK, Cat. #ab76024
CDK2, human	Rabbit, monoclonal	—	1 : 2,000	Abcam, UK, Cat. #ab32147
CDK4, human	Rabbit, monoclonal	—	1 : 2,000	Abcam, UK, Cat. #ab124821
CDK6, human	Rabbit, monoclonal	—	1 : 1,000	Abcam, Cat. #ab124821
Cleaved caspase-3, human	Rabbit, monoclonal	1 : 200	—	Cell Signaling Technology, USA, Cat. #9664
Cleaved caspase-7, human	Rabbit, monoclonal	1 : 200	—	Cell Signaling Technology, USA, Cat. #8438
Cleaved caspase-9, human	Rabbit, monoclonal	1 : 200	—	Cell Signaling Technology, USA, Cat. #7237
FOXO3a, human	Rabbit, monoclonal	—	1 : 1,000	Abcam, UK, Cat. #ab53287
Ki-67, human	Rabbit, monoclonal	1 : 200	—	Novus Biologicals, USA, Cat. #NBP2-19012
OCT-4, human	Mouse, monoclonal	1 : 200	—	Novus Biologicals, USA, Cat. #NB110-90606
p53 (acetyl K370), human	Rabbit, monoclonal	—	1 : 1,000	Abcam, UK, Cat. #ab183544
PPARγ, human	Rabbit, monoclonal	—	1 : 1,000	Abcam, UK, Cat. #ab45036
SIRT1, human	Rabbit, monoclonal	1 : 400	1 : 1,000	Cell Signaling Technology, USA, Cat. #9475
SOD2, human	Rabbit, monoclonal	—	1 : 1,000	Abcam, UK, Cat. #ab68155
β-Actin, human	Mouse, monoclonal	—	1 : 2,000	Santa Cruz Biotechnology Inc., Dallas, TX., USA, Cat. #sc-47778
E-Cadherin, human	Rabbit, monoclonal	1 : 200	—	Cell Signaling Technology, USA, Cat. #3195
N-Cadherin, human	Rabbit, monoclonal	1 : 200	—	Cell Signaling Technology, USA, Cat. #13116