BioMed Research International / 2017 / Article / Tab 3

Research Article

Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways

Table 3

List of primary antibodies.

AntigensSpecies antibodies raised inDilution (IF)Dilution (WB)Supplier

Acetyllysine, acetylated KLH conjugatesRabbit, polyclonal1 : 500Abcam, UK, Cat. #ab80178
Catalase, humanRabbit, monoclonal1 : 2,000Abcam, UK, Cat. #ab76024
CDK2, humanRabbit, monoclonal1 : 2,000Abcam, UK, Cat. #ab32147
CDK4, humanRabbit, monoclonal1 : 2,000Abcam, UK, Cat. #ab124821
CDK6, humanRabbit, monoclonal1 : 1,000Abcam, Cat. #ab124821
Cleaved caspase-3, humanRabbit, monoclonal1 : 200Cell Signaling Technology, USA, Cat. #9664
Cleaved caspase-7, humanRabbit, monoclonal1 : 200Cell Signaling Technology, USA, Cat. #8438
Cleaved caspase-9, humanRabbit, monoclonal1 : 200Cell Signaling Technology, USA, Cat. #7237
FOXO3a, humanRabbit, monoclonal1 : 1,000Abcam, UK, Cat. #ab53287
Ki-67, humanRabbit, monoclonal1 : 200Novus Biologicals, USA, Cat. #NBP2-19012
OCT-4, humanMouse, monoclonal1 : 200Novus Biologicals, USA, Cat. #NB110-90606
p53 (acetyl K370), humanRabbit, monoclonal1 : 1,000Abcam, UK, Cat. #ab183544
PPARγ, humanRabbit, monoclonal1 : 1,000Abcam, UK, Cat. #ab45036
SIRT1, humanRabbit, monoclonal1 : 4001 : 1,000Cell Signaling Technology, USA, Cat. #9475
SOD2, humanRabbit, monoclonal1 : 1,000Abcam, UK, Cat. #ab68155
β-Actin, humanMouse, monoclonal1 : 2,000Santa Cruz Biotechnology Inc., Dallas, TX., USA, Cat. #sc-47778
E-Cadherin, humanRabbit, monoclonal1 : 200Cell Signaling Technology, USA, Cat. #3195
N-Cadherin, humanRabbit, monoclonal1 : 200Cell Signaling Technology, USA, Cat. #13116

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