Figure 3: T. vaginalis initially activated NF-κB in SiHa cells, followed by a decline in NF-κB levels. (a) T. vaginalis transiently induced NF-κB p65 nuclear translocation in SiHa cells; this was followed by a dramatic decline at 4 h after treatment. (b) NF-κB activity of T. vaginalis-treated SiHa cells were significantly reduced in a parasite-dose-dependent manner at 4 h posttreatment. The experiment was repeated three times with similar results. versus SiHa cell control, versus SiHa cell control.