The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALB/c Mice and Cytotoxicity In Vitro

Figure 3

IgG Isotypes antibodies induced by xanthan gum. BALB/c mice were subcutaneously immunized twice at a two-week interval with OVA (10 μg) alone as a control or in combination with Alhydrogel (15%) or xanthan (0.5%). A group immunized with saline solution was used as control. Blood was collected on days 0, 14, and 28 after immunization. The responses of the IgG isotypes (IgG1, IgG2a, IgG2b, and IgG3) and IgG1/2a ratio at day 28 were determined by ELISA with serum dilution 1 : 100 and humoral response compared to the control group (OVA-saline). The values shown are the mean ± SD of three independent experiments. (, ).