CYLD Deubiquitinase Negatively Regulates High Glucose-Induced NF-κB Inflammatory Signaling in Mesangial Cells

Figure 1

CYLD protein and mRNA expression after high glucose challenge for various times and various glucose concentrations determined by Western blot and RT-PCR. (a) GMCs (SV40 MES 13 and HBZY-1) were treated with 30 mmol/L glucose for 6, 12, 24, 48, and 24 h, and Western blot and RT-PCR were performed to detect the expression of CYLD. (b) GMCs (SV40 MES 13 and HBZY-1) were treated with the indicated concentrations of glucose or mannitol for 24 h. The gray graph shows the relative statistical values for CYLD protein and mRNA expression in each group. The data were normalized to GAPDH/β-actin and are expressed as mean ± SD; compared with NC group; compared with 30 mmol/L glucose (HG3) group.

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