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BioMed Research International
Volume 2017, Article ID 4018562, 10 pages
https://doi.org/10.1155/2017/4018562
Research Article

The Potential of a Brown Microalga Cultivated in High Salt Medium for the Production of High-Value Compounds

1Laboratory of Enzyme Engineering and Microbiology, University of Sfax, National Engineering School of Sfax, BP 1173, 3000 Sfax, Tunisia
2Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktonology, Department of Life Sciences, Sfax University, Soukra Km 3.5, BP 1171, 3000 Sfax, Tunisia

Correspondence should be addressed to Alya Sellami-Kamoun; nt.tenut@ayla_nuomak

Received 9 February 2017; Accepted 27 April 2017; Published 22 May 2017

Academic Editor: María M. Yust

Copyright © 2017 Saoussan Boukhris et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Amphora sp. was isolated from the Sfax Solar Saltern and cultivated under hypersaline conditions. It contains moderate rates of proteins, lipids, sugars, and minerals and a prominent content of bioactive compounds: polyphenols, chlorophyll a, carotenoids, and fatty acids. The analysis of fatty acids with GC/MS showed that the C16 series accounted for about 75% of Amphora sp. lipids. Saturated fatty acids whose palmitic acid was the most important (27.41%) represented 41.31%. Amphora sp. was found to be rich in monounsaturated fatty acids with dominance of palmitoleic acid. It also contains a significant percentage of polyunsaturated fatty acids with a high amount of eicosapentaenoic acid (2.36%). Among the various solvents used, ethanol at 80% extracted the highest amounts of phenols and flavonoids that were 38.27 mg gallic acid equivalent and 17.69 mg catechin equivalent g−1 of dried extract, respectively. Using various in vitro assays including DPPH and ABTS radicals methods, reducing power assay, and β-carotene bleaching assay, the 80% ethanolic extract showed high antioxidant activity. A strong antibacterial activity was checked against Gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus) and Gram-negative bacteria (Klebsiella pneumoniae and Salmonella enterica). These results are in favor of Amphora sp. valorization in aquaculture and food and pharmaceutical industries.