Salinomycin Exerts Anticancer Effects on PC-3 Cells and PC-3-Derived Cancer Stem Cells In Vitro and In Vivo

Figure 6

Salinomycin targets PC-3-derived CSCs to exert antitumor effects. (a) ALDH activity of PC-3 cells was analyzed by flow cytometry after salinomycin (5.0 μM) or paclitaxel (100 nM) treatment. Diethylaminobenzaldehyde (DEAB), an ALDH inhibitor, was used as a negative control for ALDH staining. (b) The histogram shows the percentage of ALDH-positive cells. The data are presented as means ± SDs (). and versus DMSO. (c) The PC-3-derived CSCs mammospheres cultured in ultralow attachment plates and incubated with salinomycin (5.0 μM) or paclitaxel (100 nM) for 7 days. Scale bars: 50 μm. (d) The histogram shows the mammospheres formation normalized to control. The data are presented as means ± SDs (). and versus control.

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