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BioMed Research International
Volume 2017 (2017), Article ID 4107587, 11 pages
https://doi.org/10.1155/2017/4107587
Research Article

Preservation of Myocardial Perfusion and Function by Keeping Hypertrophied Heart Empty and Beating for Valve Surgery: An In Vivo MR Study of Pig Hearts

1Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, China
2National Research Council of Canada, 435 Ellice Avenue, Winnipeg, MB, Canada R3B 1Y6
3Department of Physiology, Faculty of Medicine, University of Manitoba, 727 McDermot Avenue, Winnipeg, MB, Canada R3E 3P5
4Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, 753 McDermot Avenue, Winnipeg, MB, Canada R3E 0T6
5Division of Cardiac Surgery, University of Alberta Hospital, 8440-112 Street, Edmonton, AB, Canada T6G 2B7
6Cardiac Science Program, Institute of Cardiovascular Science, St. Boniface General Hospital, 409 Tache Avenue, Winnipeg, MB, Canada R2H 2A6

Correspondence should be addressed to Jian Wang; moc.621@0301gnawnaij and Ganghong Tian; ac.cg.crnc-crn@nait.gnohgnag

Received 2 August 2016; Revised 13 December 2016; Accepted 29 December 2016; Published 20 March 2017

Academic Editor: Dirk Bandorski

Copyright © 2017 Jian Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. Normothermic hyperkalemic cardioplegia arrest (NHCA) may not effectively preserve hypertrophied myocardium during open-heart surgery. Normothermic normokalemic beating perfusion (NNBP), keeping hearts empty-beating, was utilized as an alternative to evaluate its cardioprotective role. Materials and Methods. Twelve hypertrophied pig hearts at 58.6 ± 7.2 days after ascending aorta banding underwent NNBP and NHCA, respectively. Near infrared myocardial perfusion imaging with indocyanine green (ICG) was conducted to assess myocardial perfusion. Left ventricular (LV) contractile function was assessed by cine MRI. TUNEL staining and western blotting for caspase-3 cleavage and cardiac troponin I (cTnI) degradation were conducted in LV tissue samples. Results. Ascending aortic diameter was reduced by at approximately fifty-eight days after banding. LV wall thickness was significantly higher in aorta banding than in sham operation. Myocardial blood flow reflected by maximum ICG absorbance value was markedly higher in NNBP than in NHCA. The amount of apoptotic cardiomyocyte was significantly lower in NNBP than in NHCA. NNBP alleviated caspase-3 cleavage and cTnI degradation associated with NHCA. NNBP displayed a substantially increased postoperative ejection fraction relative to NHCA. Conclusions. NNBP was better than NHCA in enhancing myocardial perfusion, inhibiting cardiomyocyte apoptosis, and preserving LV contractile function for hypertrophied hearts.