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BioMed Research International
Volume 2017, Article ID 4151594, 10 pages
Research Article

Xanthine-Catechin Mixture Enhances Lithium-Induced Anti-Inflammatory Response in Activated Macrophages In Vitro

1Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil
2Postgraduate Program of Gerontology, Federal University of Santa Maria, Santa Maria, RS, Brazil
3Open University of the Third Age, State University of Amazonas, Manaus, AM, Brazil
4Brain Institute, Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil
5Lutheran University of Brazil, Santa Maria, RS, Brazil

Correspondence should be addressed to Ivana Beatrice Mânica da Cruz; moc.liamg@msfu.anavi

Received 14 July 2017; Revised 19 September 2017; Accepted 11 October 2017; Published 8 November 2017

Academic Editor: Gjumrakch Aliev

Copyright © 2017 Fernanda Barbisan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lithium (Li) is a chemical element used for treating and preventing bipolar disorder (BD) and exerts positive effects such as anti-inflammatory effects as well as undesirable side effects. These effects of Li can be influenced by interaction with some nutritional elements. Therefore, we investigated the potential effects of xanthine (caffeine and theobromine) and catechin molecules present in some food beverages broadly consumed worldwide, such as coffee and tea, on Li-induced anti-inflammatory effects. In the present study, we concomitantly exposed RAW 264.7 macrophages to Li, isolated xanthine and catechin molecules, and a xanthine-catechin mixture (XC mixture). We evaluated the effects of these treatments on cell proliferation, cell cycle progression, oxidative and antioxidant marker expression, cytokine levels, gene expression, and GSK-3β enzyme expression. Treatment with the XC mixture potentialized Li-induced anti-inflammatory effects by intensification of the following: GSK-3β inhibitory action, lowering effect on proinflammatory cytokines (IL-1β, IL-6, and TNFα), and increase in the levels of IL-10 that is an anti-inflammatory cytokine. Despite the controversial nature of caffeine consumption by BD patients, these results suggested that consumption of caffeine, in low concentrations, mixed with other bioactive molecules along with Li may be safe.