The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse

Figure 4

Proliferation state of follicles. (A) Immunohistochemical localization of PCNA. Expression of PCNA in control (a) and NAC treated group (c) was at similar level, and sections from the mice exposed to X-irradiation represented weak expression of PCNA (b). PCNA expression of the NAC pretreatment group showed similar level to the control group (d). Scale bar, 20 μm. (B) Quantitative image analysis for IHC staining expressed as mean density in each group. Each column represents the mean ± SD of at least three independent experiments ( versus control; versus X-irradiation group). (C) Detection of PCNA by western blot images was representative of at least three independent experiments. (D) Quantitative image analysis for western blot. Each column represents the mean ± SD of at least three independent experiments ( versus control; versus X-irradiation group).