Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis

Figure 1

Representative 2D gel images showing the differentially expressed proteins in the secretome of cervical cancer cell lines versus the normal cancer cell line HCK1T. Representative 2D gels of each cancer cell line secretome (left) and HCK1T (right). Differentially expressed spots (over 2-fold), four gels per category corresponding to four biological replicates, are shown. (a) SiHa versus HCK1T comparison, (b) HeLa versus HCK1T comparison, (c) C33A versus HCK1T comparison. For the secretome analysis, 60 μg of total protein was analyzed, using 7 cm nonlinear strips, pH range 3–10, and spot detection was performed by Coomassie Colloidal Blue staining. Protein identification was conducted by MALDI-TOF-MS.

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