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BioMed Research International
Volume 2017 (2017), Article ID 4248756, 12 pages
Research Article

A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray

1Institute for Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Insel Riems, Greifswald, Germany
2Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Jena, Germany
3Diagnostic Laboratory, Department of Clinical Sciences, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece

Correspondence should be addressed to Martin Eiden

Received 20 December 2016; Accepted 7 February 2017; Published 24 May 2017

Academic Editor: Satish B. Nimse

Copyright © 2017 Ariel Vina-Rodriguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S01: The visual program PanFlavExpStdSampl. Schematic workflow—the visual program PanFlavExpStdSampl for the Orange software package. The scheme permits an interactive import of the experiments used as standards (known samples), which are being subsequently used to identify new or unknown samples. Figure S02: WNV titration. Figure S03: USUV titration. Table S01: Flavivirus-specific oligonucleotide probes, including spot number, position, probe name and probe sequence. Table S02: Quantification of flaviviruses in samples from the QCMD-2013 ring trial using the new Flavivirus RT-qPCR and a calibrated WNV NY99 RNA standard curve.

  1. Supplementary Material