Research Article
A Novel -72 (T→A) β-Promoter Mutation Causing Slightly Elevated HbA2 in a Vietnamese Heterozygote
Table 1
Tabulated details of PCR conditions.
| | Primer pair | Target region | MgCl2 (mM) | dNTPs (μM) | Thermal conditions |
| | β1-β2 | HBB (5′ UTR to exon 2) | 3.5 | 250 | (94°C-65°C-72°C) × 35 cycles | | β7-β8 | HBB (exon 2 to IVS 2) | 3.0 | 250 | (94°C-60°C-72°C) × 35 cycles | | β9-β10 | HBB (IVS 2 to 3′ UTR) | 1.5 | 250 | (94°C-55°C-72°C) × 35 cycles | | β_KpnI-β _XhoI | HBB (promoter) | 4.0 | 300 | (94°C-66°C-72°C) × 35 cycles | | HS2_BamHI-HS2_SalI | β-LCR (HS2) | 4.0 | 200 | (94°C-64°C-72°C) × 35 cycles |
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Each thermal profile was preceded by a denaturation of 94°C for 3 min and followed by an additional extension of 74°C for 4 min.
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