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BioMed Research International
Volume 2017 (2017), Article ID 4565960, 7 pages
Research Article

Analysis of Draft Genome Sequence of Pseudomonas sp. QTF5 Reveals Its Benzoic Acid Degradation Ability and Heavy Metal Tolerance

1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
2Shanghai Majorbio Bio-Pharm Biotechnology Co., Ltd, Shanghai, China
3Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China

Correspondence should be addressed to Nan Jiang

Received 16 June 2017; Revised 4 October 2017; Accepted 1 November 2017; Published 15 November 2017

Academic Editor: Carla Renata Arciola

Copyright © 2017 Yang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pseudomonas sp. QTF5 was isolated from the continuous permafrost near the bitumen layers in the Qiangtang basin of Qinghai-Tibetan Plateau in China (5,111 m above sea level). It is psychrotolerant and highly and widely tolerant to heavy metals and has the ability to metabolize benzoic acid and salicylic acid. To gain insight into the genetic basis for its adaptation, we performed whole genome sequencing and analyzed the resistant genes and metabolic pathways. Based on 120 published and annotated genomes representing 31 species in the genus Pseudomonas, in silico genomic DNA-DNA hybridization (<54%) and average nucleotide identity calculation (<94%) revealed that QTF5 is closest to Pseudomonas lini and should be classified into a novel species. This study provides the genetic basis to identify the genes linked to its specific mechanisms for adaptation to extreme environment and application of this microorganism in environmental conservation.