A Simple Model for Inducing Optimal Increase of SDF-1 with Aminoglycoside Ototoxicity

Figure 1

The study design for the mice model showing optimal increase of SDF-1 with aminoglycoside ototoxicity. (a) Total population involving 63 male C57BL/6 mice (including 18 control mice). Mice were treated according to the following protocol: to record auditory brainstem responses (ABRs) 24 h after the second LPS injection, mice were injected with kanamycin (420, 550, and 600 mg/kg) and, 30 min later, with furosemide (130 mg/kg). (b) Auditory brainstem responses (ABRs) were tested on days 3, 5, 7, and 14 after kanamycin-furosemide administration. On the 14th day, fixative was perfused through the heart and cochleas were harvested for histologic analysis.

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