Pharmacokinetics, Tissue Distribution, and Metabolism Study of Icariin in Rat

Table 6

Characterization of metabolites of icariin in rat feces.

Number	Compound	RT	Formula	Observed mass	RDB	Error	LC/MS^n data
						(ppm)
	Icariin	55.19	C33H39O15	675.22888	14.5	0.790	MS^2   367 (100), 352 (25); MS^3   352 (100); MS^4   323 (30), 309 (100), 296 (45), 281 (65), 268 (50), 255 (20)
	Epimedin A isomer	50.38	C39H49O20	837.2819	15.5	0.824	MS^2   790 (50), 367 (100); MS^3   352 (100), 297 (20)
	Sagittatoside B isomer	50.44	C32H37O14	645.2186	14.5	1.314	MS^2   481 (20), 352 (100); MS^3   323 (40), 309 (60), 296 (90), 281 (100), 268 (60)
	Baohuoside II	52.78	C26H27O10	499.1605	13.5	1.295	MS^2   353 (100); MS^3   325 (20), 298 (40), 284 (100), 255 (30)
	Icariin glucuronide	54.04	C33H37O16	689.2084	15.5	1.202	MS^2   367 (100), 321 (60); MS^3   352 (100)
	Hydroxyl icariside II	54.12	C27H29O11	529.1716	13.5	1.132	MS^2   501 (20), 382 (100), 367 (40); MS^3   367 (100), 339 (10)
	Sagittatoside B	57.43	C32H37O14	645.2184	14.5	1.020	MS^2   367 (100), 352 (30); MS^3   352 (100)
	2′′-O-Rhamnosyl-icariside II	57.56	C33H39O14	659.2344	14.5	1.529	MS^2   366 (100), 351 (20), 323 (15); MS^3   351 (100), 323 (80), 311 (60), 305 (20)
	Icariside II	61.46	C27H29O10	513.1760	13.5	0.851	MS^2   366 (100); MS^3   351 (100), 323 (80), 311 (50), 305 (20)
	Hydroxyl icaritin	62.33	C21H19O7	383.1137	12.5	3.160	MS^2   365 (100), 355 (60), 339 (40); MS^3   350 (100), 321 (20), 230 (40)
	Desmethyl icaritin	66.36	C20H17O6	353.1024	12.5	1.119	MS^2   298 (100), 219 (10); MS^3   279 (60), 269 (40), 253 (100), 241 (30), 164 (70)
	Icaritin	82.65	C21H19O6	367.1183	12.5	1.921	MS^2   352 (100), 309 (20), 297 (20); MS^3   309 (100), 297 (60)

Compared with the reference standard. Observed in rat feces after oral administration. Observed in rat feces after intramuscular administration.