Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in<i> E. coli</i> via Mutation of Free Cysteine Residues

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr class="thead"><td class="align_left"> </td><td class="align_center">wtSOD1</td><td class="align_center">mhSOD1/C6S</td><td class="align_center">mhSOD1/C111S</td><td class="align_center">mhSOD1/C6S/C111S</td></tr><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td class="align_left">Soluble expression</td><td class="align_center">1.00</td><td class="align_center">0.58 ± 0.02↓</td><td class="align_center">2.35 ± 0.07↑</td><td class="align_center">2.20 ± 0.10↑</td></tr><tr><td class="align_left">Activity yield</td><td class="align_center">1.00</td><td class="align_center">0.26 ± 0.02↓</td><td class="align_center">3.25 ± 0.07↑</td><td class="align_center">3.19 ± 0.04↑</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td>The ratios of mhSOD1s to wtSOD1 in soluble expression and yields were calculated. ↑ presents increase and ↓ presents decrease when compared with the wtSOD1.<br/></td></tr></table>

Ratios of mhSOD1s to wtSOD1 in soluble expression and yields.

BioMed Research International

tab1

Table 1

Table 1: Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in<i> E. coli</i> via Mutation of Free Cysteine Residues 

BioMed Research International

Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues

Table 1